Introduction: Candida albicans (C. Albicans) is an oral cavity commensal organism that can shift into a
pathogenic form under certain conditions. Nowadays, natural substances used as an alternative for a new
antifungal agent. Snakehead fish oil extracts have been reported to have antifungal effect i.e; unsaturated fatty
acids such as Omega-3 and Omega-6. The purpose of this study was to investigate the antifungal activity of
snakehead fish oil extracts against C. Albicans. Materials and Methods: An in vitro laboratory study with a posttest control group design was established. To produce the snakehead fish oil extracts, wet rendering method was
used. The snakehead fish oil extracts then tested with microdilution method to determine Minimum Inhibitory
Concentration (MIC) and Minimum Fungicidal Concentration (MFC) against C. Albicans. An 0,062-32 μl/ml
concentrations used for the microdilution test and 2-32 μl/ml for the disc-diffusion test. Nystatin (100 μg/ml) was
used as the positive control. Results: There were visible growths and no inhibition zone in all concentrations of
snakehead fish oil extracts. Conclusion: 0.062-32 μl/ml concentrations of Snakehead fish oil extracts did not have
the abilities to decrease the growth of C. albicans.